The Effects of Raddeanin A on Human Gastric Cancer BGC-823 Cell Cycle and STAT3 Signaling Pathway

OBJECTIVE To investigate the effects of Raddeanin A（RA） on cell cycle and STAT3 signaling pathway in human gastric cancer cell line BGC-823. METHODS Effects of different concentrations of RA on proliferation of BGC-823 cell line were investigated with MTT assay; flow cytometry was used to detect the cell cycle inhibition; wounding heal assay was used to measure the influence of migration ability of RA on BGC-823 cell; the effect of STAT3 signaling pathway of RA on BGC-823 cell was measured by Western blot. RESULTS After the BGC-823 cells were treated by RA for 12， 24， 48h， compared with control group， RA significantly inhibited the proliferation of BGC-823 cell (P<0.01). The inhibitive effects presented a dose and time-dependent manner. Flow cytometry demonstrated that RA blocked the cell cycle in S phase; wounding heal assay revealed that RA inhibited the migration of BGC-823 cell. Western blot results showed that RA down-regulated the expression of phosphorylated STAT3 (p-STAT3). CONCLUSION RA can effectively inhibit the proliferation of BGC-823 cell， block its cell cycle in S phase and inhibit the expression of p-STAT3.