Preparation Optimization of Baicalein Nano-micelles by Box-Behnken Design-Response Method

OBJECTIVE To prepare the Nanomicelles of Baicalein-polyethylene glycol-12 hydroxy stearic acid ester-phospholipids for improving its solubility， and to optimize its technology. METHODS Baicalein-solutol HS15- phospholipid (BA-Sol-Pls) were prepared by film-dispersion method. Box-behnken design was employed， with the amount of ethanol (X₁)， concentration of solutol (X₂) and phospholipids (X₃) as the investigation object. Particle size analyzer was used to investigate the particle size and Zeta potential of Nanomicelles. The encapsulation efficiency and drug loading of micelles were determined by Ultracentrifugation. RESULTS The BA-Sol-Pls prepared by optimal formulation were well-distributed. The average particle diameter was (410±5.98) nm， Zeta potential was -(21±0.92) mV. The encapsulation efficiency was 90.38% and the drug-loading rate was 5.35%. CONCLUSION The box-behnken design was effective and suitable for optimization the preparation technology of baicalein nano micelles.