Formulationandprocess Optimization of Puerarin-loaded PEG-PLGA Nanoparticles Modified by Hyaluronic Acid and Its in Vitro Evaluation

OBJECTIVE With PEG-PLGA as the carrier， and the preparation of hyaluronic acid modified kudzu root element PEG-PLGA nanoparticles by nanoprecipitation method， to optimize the preparation prescription， and preliminary evaluation of its properties in vitro. METHODS With PEG PLGA as the carrier， Hyaluronic acid as surface modification agent， preparation of hyaluronic acid modified kudzu root element PEG-PLGA HA/Pue-NPs by nanoprecipitation method: design and optimize the prescription by Orthogonal experimental， characterize its in vitro properties， examine the in vitro drug release behavior of HA/Pue-NPs. RESULTS Prepared drug-loading nanoparticles has the spherical appearance. The average particle size and Zeta potential are (88.9±2.2 )nm、（-21.9± 0.54）mV respectively， drug loadings and the coating rate are 6.75%， 78.52%. In vitro drug release test shows that the drug-loading nanoparticles release slowly， the accumulative release rate is 65.8% within 24h. CONCLUSION The particle size of HA/Pue-NPs is consistent， good in vitro properties and certain slow-release characterized.