Metabonomics Research on the Urine of Model of Dampness Stagnancy due to Spleen Deficiency Based on Liquid Mass Spectrometry

OBJECTIVE In metabonomics method based on liquid mass combination of dampness stagnancy due to spleen deficiency model and normal control group rats urine， To explore the differences metabolites in the urine of the model rats. METHODS The model of spleen deficiency and dampness stagnancy in rats was induced with high-fat and low-protein diet plus exhaustive swimming methods. Using high performance liquid chromatography(HPLC)-quadrupole-time of flight tandem mass spectrometer technique to detect the small molecule metabolites in urine samples in the 2 groups， Metabolic fingerprint as principal component analysis and partial least squares discriminant analysis， find out the differences of characteristics of metabolites. RESULTS Compared with the control group， the urine of rats in model group identified 6 different metabolites， respectively: alpha keto glutarate， citrulline， lactic acid， glutamine， citric acid， serotonin. CONCLUSION Dampness stagnancy due to spleen deficiency model rats body sugar and protein metabolism in disorder， energy supply， gastrointestinal function drops， antioxidant ability， its mechanism may be related to the change of the small molecule metabolites.