YANGMei, ZHAORuiting, XULi, BIANYong. Mechanism of Wenwei Tiaochang Granule on Rats with Diarrhea-Predominant Irritable Bowel Syndrome[J]. Journal of Nanjing University of traditional Chinese Medicine, 2016, 32(4): 362-366.
Citation: YANGMei, ZHAORuiting, XULi, BIANYong. Mechanism of Wenwei Tiaochang Granule on Rats with Diarrhea-Predominant Irritable Bowel Syndrome[J]. Journal of Nanjing University of traditional Chinese Medicine, 2016, 32(4): 362-366.

Mechanism of Wenwei Tiaochang Granule on Rats with Diarrhea-Predominant Irritable Bowel Syndrome

More Information
  • OBJECTIVE To explore the effects and mechanisms of Wenwei Tiaochang Granule on diarrhea-predominant irritable bowel syndrome rats. METHODS 60 SD rats, were randomly divided into control group, model group, positive drug group, and low, middle and high dose groups of Wenwei Tiaochang Granule. In order to make the rats get diarrhea-predominant irritable bowel syndrome, the rats were administrated with senna, bound limbs and clipped tails for 14d. After 14d of administration with positive drug and Wenwei Tiaochang Granule, the blood, jejunum, colon, thymus and spleen were collected. Motilin assay kit was used to detect motilin (MTL) content in plasma and jejunum. Use the weight of thymus and spleen to calculate their organ index. Calcitonin gene-related peptide(CGRP) and substance P (SP) mRNA level and protein expression level was detected by RT-PCR and Western-blot in colonic tissues. RESULTS Compared with the control group, the growth rate and food intake increased slower in model group, at the same time, the thymus index, spleen index and MTL content in plasma and jejunum decreased significantly, while the CGRP and SP mRNA and protein expression level of colon increased significantly (P<0.05~0.01). Compared with the model group ,the middle and high dose groups of Wenwei Tiaochang Granule could significantly increase the thymus index, spleen index and MTL content in plasma and jejunum, and decrease CGRP and SP mRNA and protein expression level of colon(P<0.05~0.01). CONCLUSION By regulating the content of MTL and the expression level of CGRP and SP mRNA and protein, Wenwei Tiaochang Granule can be used to treat diarrhea-predominant irritable bowel syndrome.
  • [1]
    BELLINI M, TOSETTI C, COSTA F, et al. The general practitioner's approach to irritable bowel syndrome: from intention to practice[J]. Dig Liver Dis, 2005, 37(12): 934-939.
    [2]
    VIDELOCK EJ, CHANG L. Irritable bowel syndrome: current approach to symptoms, evaluation, and treatment[J]. Gastroenterol Clin North Am, 2007, 36(3): 665-685, x.
    [3]
    LONGSTRETH FG. Functional bowel disorders[J]. Am J Gastroenterol, 2014, 109(Suppl 2): S527-S544.
    [4]
    BURBIGE EJ. Irritable bowel syndrome: diagnostic approaches in clinical practice[J]. Clin Exp Gastroenterol, 2010, 3: 127-137.
    [5]
    NELLESEN D, CHAWLA A, OH DL, et al. Comorbidities in patients with irritable bowel syndrome with constipation or chronic idiopathic constipation: a review of the literature from the past decade[J]. Postgrad Med, 2013, 125(2): 40-50.
    [6]
    CAMILLERI M, LASCH K, ZHOU W. Irritable bowel syndrome:methods,mechanisms,and pathophysiology. The confluence of increased permeability,inflammation,and pain in irritable bowel syndrome[J]. Am J Physiol Gastrointest Liver Physiol, 2012, 303(7): 775-785.
    [7]
    唐洪梅,房财富,廖小红,等.神经肽Y和5-羟色胺在腹泻型肠易激综合征模型大鼠中表达的研究[J].中国药理学通报,2012, 28(7):916-920.
    [8]
    TANG HM, FANG CF, LIAO XH, et al. Expression of NPY and 5-HT in rat model of diarrhea-predominant irritable bowel syndrome[J]. Chin Pharmacol Bull, 2012, 28(7): 916-920.
    [9]
    刘美荣,肖瑞飞,彭芝配,等.针刺“足三里”“太冲”穴对腹泻型肠易激综合征大鼠胃肠激素的影响[J].针刺研究, 2012,37(5):363-368.
    [10]
    LIU MR, XIAO RF, PENG ZP. et al. Effect of acupuncture at "Zusanli"(ST 36) and "Taichong"(LR 3) on gastrointestinal hormone levels in rats with diarrhea type irritable bowel syndrome[J].Acup Res, 2012,37(5):363-368.
    [11]
    张峰,袁川评,柳巨雄,等.SP和CGRP在炎症性肠病大鼠结肠中的表达规律[J].中国比较医学杂志,2011, 21(12):26-30.
    [12]
    ZHANG F, YUAN CP, LIU JX, et al. SP and CGRP in the occurrence and development of inflammatory bowel disease[J]. Chin J Comp Med, 2011, 21(12): 26-30.
    [13]
    朱方成. 大鼠GAPDH、βactin mRNA降解与死亡时间推断的相关性研究[D]. 武汉:华中科技大学, 2006.
    [14]
    ZHU FC. Study on relationship between GAPDH, βactin mRNA degradation and the postmortem interval in rats[D]. Wuhan:Huazhong University of Science, 2006.

Catalog

    Article Metrics

    Article views (949) PDF downloads (621) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return