Explore the Mechanism of Chinese Medicine Theory “Salt-hurt-blood” Based on High Salt-induced Kidney Injury in SHR Mice

OBJECTIVE To study the related basic research of high salt-induced kidney injury in SHR rats， and discuss the salt-hurt-blood connotation of traditional Chinese medicine. METHODS WKY rats were set as the normal group. SHR were randomly divided into 2 groups: normal group (SHR) and high-salt feed group (SHR+HS). SHR+HS group rats were given 4% high salt diet from the 10th week. Urine trace albumin and NAG in urine were detected every two weeks. On the 20th week， the hemorheology indexes and coagulation four indices were measured. The rat kidney was divided into two parts. One was fixed with formalin and detected with HE; the other part was used to detect ICAM-1， VCAM-1 and IL-6 expressions. RESULTS After feeding high-salt feed for 6 weeks， urine trace albumin and NAG increased significantly(P<0.05). On the 20th week， the whole blood viscosity and blood plasma viscosity in SHR+HS group were significantly(P<0.05) greater than SHR group. APTT and PT were significantly reduced and FIB significantly increased(P<0.05). Renal pathology index in SHR+HS group was higher than SHR group， and the kidney inflammation factors ICAM 1， VCAM 1 and IL-6 increased(P<0.05). CONCLUSION High salt can increase the whole blood viscosity， disorder APTT， PT and FIB， increase the expression of ICAM 1， VCAM 1 and IL-6， and strengthen the kidney injury of SHR rats， which might partly explain the scientific connotation of "salt-hurt-blood" and "salty flavor entering the kidney".