Intervention Effect of Taishan Panshi San on Early Pregnancy Loss in PCOS Rats with Ovulation Induction

OBJECTIVE To investigate the possible mechanism of early pregnancy loss in PCOS rats with ovulation induction and the intervention effect of Taishan Panshi San on pregnancy loss. METHODS To select normal pregnant rats mated with male rats as group A. The PCOS rats model by dehydroepiandrosterone(DHEA) was stimulated ovulation by clomiphene(CC)，then mated with male rats to establish the rat model of pregnancy.They were randomly divided into two groups：treated by normal saline (group B) and by Taishan Panshi San (group C). According to the pregnancy third days， fourth days， fifth days， group B was divided into group B3， group B4， group B5. To detect the mRNA and protein expression of endometrium HOXA10， integrinαvβ3， EMX2， LIF by RT-PCR and Western blot in each group. RESULTS In group B of PCOS Pregnant rats with ovulation induction：The expression of mRNA HOXA10 in Group B4 was higher than group B3 and group B5（P<0.05~0.01），the expression of mRNA integrinαvβ3 was significantly higher than group B3 and group B5（P<0.01），but the expression of mRNA EMX2 was lower than group B5（P<0.05）；the expression of mRNA LIF was highest in group B3， higher than group B4（P<0.05）。In the planting window period:the mRNA expression of endometrium HOXA10， integrinαvβ3， LIF in Group B5 were significantly lower than Group A and Group C (P<0.05~0.01)；the expression of endometrium EMX2 in Group A was significantly lower than Group B5 and Group C (P<0.01)；the protein expression of endometrium HOXA10， integrinαvβ3， LIF in Group B5 were significantly lower than Group A and Group C ，but the protein expression of EMX2 higher than Group A and Group C (P<0.01). CONCLUSION PCOS rats with ovulation induction occurred early pregnancy loss may be related to the low expression of endometrial HOXA10，integrinαvβ3， LIF and the high expression of EMX2 in the implantation window phase. This may also be related to the abnomal expression of HOXA10， integrinαvβ3， LIF， EMX2 before the planting window.Taishan Panshi San improved the endometrial receptivity and reduced the early pregnancy loss，probably by raising the expressions of endometrium HOXA10， integrinαvβ3， LIF and decreasing the expressions of EMX2 in the implantation window phase.