Study on Decoction Process and Antifatigue Effect of Polygonatum Lilium Meretrix Decoction

OBJECTIVE To determine the best water-coction extract technology of Polygonatum Lilium Meretrix Decoction (PLMD) and investigate the anti-fatigue effect of PLMD in mice. METHODS The preperation condition of PLMD was optimized by orthogonal design with extractive yield， amount of polysaccharides and taurine as index. At the same time， mice were randomly divided into six groups: normal group， model group， positive group and administrated groups. In administrated groups， mice were given different doses of PLMD. Swimming model was established after orally administrated daily for 15 days， loaded-swimming time and serum indicators were conducted to explore PLMD's antifatigue effect. RESULTS The best decoction technique was adding 10-fold of water， decocting three times 60minutes each time，in which the polysaccharides and taurine content was (79.48±7.82)， (1.95±0.02) mg/g crude drug， respectively， extractive yield was (26.82±0.53)%. The medium and high doses group of PLMD could obviously prolong the loaded-swimming time， significantly decreased LA， MDA， LDH， CK levels， and the enzyme activity of SOD was ehhanced in dose groups than in model group. CONCLUSION The PLMD’s best decoction process was identified and its antifatigue effect was also confirmed.This result provided the basis for the further development of PLMD.