Protective Effects of Lemai Granules on Myocardial Ischemia and Its Mechanisms

OBJECTIVE To observe the effect of Lemai granules on model rats with myocardial ischemia and explore its mechanism. METHODS 48 male SD rats were randomly divided into six groups: normal group， model group， positive control group （Wenxin granules group）and high， middle and low dose group (0.84， 0.42， 0.21 g/kg) of Lemai granules. Myocardial ischemia rats model was established by intraperitoneal injection of isoproterenol (ISO)， and then different doses of Lemai granules were administered intragastrically to the myocardial ischemia rats for 7 d. The changes of the ST-segment of Ⅱlead electrocardiogram (ECG) was observed. The activities of CK， LDH and SOD， the content of MDA， NO， ET， IL-1β，TNF-α and CRP in serum were measured， and changes of blood rheology were tested. RESULTS ST-segment and the activities of CK and LDH of Lemai granules groups(0.84 g/kg， 0.42 g/kg， 0.21 g/kg) were lower obviously than that of model group (P<0.01). Compared with the model group， the activities of SOD and NO in Lemai granule groups of the high and middle doses (0.84 g/kg and 0.42 g/kg) increased in various degrees， while the levels of MDA， ET， IL-1β， TNF-α and CRP were decreased (P<0.05~0.01). The blood rheology were improved significantly. CONCLUSION Lemai granules shows promising protective effects on myocardial ischemia and its mechanism may be related to antioxidant action， regulation of endothelial cells， anti-inflammatory effects and blood rheology improvement.