Effect of Shengjiang San on NF-κB Activity in LPS-activated Rat Alveolar Marcrophage Cell Line

OBJECTIVE To investigate the effect of Shengjiang San on expression of TLR4 and NF-κB in LPS-induced rat pulmonary alveolar macrophage cell. METHODS NF-κB signal pathway in NR8383 cells was stimulated with LPS. The expression level of TLR4 was measured by QRT-PCR and Western Blotting separately. NF-κB activity was detected by Dual Luciferase Reporter Gene Assay kit. The protein expression level of p-p65 was detected by Western Blotting. The mRNA level of TNF-α，A20，IL-6， IL-1β was measured by QRT-PCR， and the production of TNF-α， IL-6， IL-1β in the supernatant of NR8383 was determined with ELISA. Shengjiang San treated NR8383 cells， which were induced NF-κB signal pathway by LPS. NF-κB activity was detected by Dual Luciferase Reporter Gene Assay kit， qRT-PCR， Western Blotting and ELISA. RESULTS LPS induced TLR overpression in both mRNA and protein levels. Moreover， NF-κB signaling pathway and downstream target cytokines were increased with dose-dependent. 10 μg/mL Shengjiang San treatment abrogated the induction of LPS to TLR4 and NF-κB signaling pathway. CONCLUSION Shengjiang San significantly reduced LPS-activated NF-κB signaling pathway in NR8383.