Effects of Acupuncture Oninsulin Resistance and Morphologic Changes of Islet β-cell in Rat with Type 2 Diabetes Mellitus

OBJECTIVE To study the effects of Regulating spleen-stomach on acupuncture on insulin resistance (IR) and morphologic changes of islet β-cell in rat with type 2 diabetes mellitus (T2DM). METHODS 10 rats were randomly selected into normal group from thirty-six male Wistar clean-grade rats. The rest rats were made The T2DM rat model，which established with streptozotocin(25 mg/kg) and high fat diet. Twenty rats were made T2DM rat model successfully，which divided into acupuncture group (10 rats)and model group(10 rats)，according to Stratified randomization. Normal group were not given any treatment. Acupuncture group were treated with acupuncture that were dressed in clothes which reduce restless.and the treatment was given for continuous 4 weeks.But model group were not given any treatment. Several outcome indicators were measured such as body mass， fasting blood-glucose(FBG)， 2 hour postprandial blood glucose(P2BG)， fasting insulin(FINS)， insulin sensitivity index(ISI).After the last intervention，the rats were killed and pancreas were made HE staining. RESULTS One weeks after the intervention， body mass of acupuncture group increased significantly compared with normal group（P<0.05），which had no significantly difference with model group（P>0.05）. Body mass of model group were significantly reduced compared with normal group and acupuncture group at the four weeks after the intervention（P<0.05）. The FBG and P2BG of acupuncture group were lower than model group at the every weeks after the intervention（P<0.01）.FINS of model group and acupuncture group were higher than normal group front the intervention（P<0.01），which increased significantly compared with other groups in the ISI（P<0.01）. FINS of model group were higher than acupuncture group and normal group after the intervention（P<0.01），what is more，ISI of acupuncture group increased significantly compared with model group（P<0.05）. Acupuncture group has protective effects of morphology of islet β-cell. CONCLUSION Regulating spleen-stomach on acupuncture could reduces the level of FBG， P2BG and FINS and improves ISI and relieve IR， which maintains rats of body mass promoting restoration of islet and β-cell.