Effect of Active Components from Lycii Cortex on Proliferation of High Glucose-induced Glomerular Mesangial Cells and Extracellular Matrix

OBJECTIVE To observe the effect of active components from Lycii cortex on the proliferation of high glucose-induced glomerular mesangial cells (GMCs) and the secretion of extracellular matrix (ECM). To investigate the protective mechanism of diabetic nephropathy. METHODS GMCs were cultured and divided into high glucose group (final glucose concentration of 30 mmol/L)， blank group (final glucose concentration of 5.5 mmol/L)， betaine and kaempferol groups of 5 different concentrations of 0.01， 0.1， 1， 10， 100 μmol/L. The proliferation of high glucose-induced GMCs were observed by MTT method and secretion of ECM was detected by ELISA assay. RESULTS Different doses of betaine and kaempferol inhibited the proliferation of GMCs， and decreased the content of ECM， compared with high glucose group （P<0.05~0.01）. CONCLUSION Betaine and kaempferol can protect the high glucose-induced GMCs through inhibiting the proliferation of GMCs and decreasing the content of ECM.