Antioxidant Effects of Buxin Ruanmai Granules Serum on AngⅡ-induced Human Umbilical Vein Endothelial Cells

OBJECTIVE To observe the effects of Buxin Ruanmai granules (BXRM) serum on AngiotensinⅡ（AngⅡ）-induced oxidative damage related indicators， such as SOD，NADPH ，MDA，p40^phox，p47^phox and p67^phox， in HUVEC. METHODS Rats were randomly divided into normal group，Valsartan group and BXRM group，each group of 10， and lavaged for 6 weeks. Drug serum were prepared from arterial blood. Then 10^-6 mol/L Ang Ⅱ induced HUVEC damage and apoptosis model of human umbilical vein endothelial cells was established， and drug serum was used to intervene. SOD were determined by xanthinoxidase. NADPH was determined by ELISA. MDA was determined by thibabituric acid method. The expression of p40^phox， p47^phox and p67^phox in cell membrane and cytoplasm of HUVEC were determined by Western blot． RESULTS After HUVECs were damaged by 10^-6  mol/L Ang Ⅱ，the content of SOD decreased ， NADPH and MDA increased ， and p40^phox， p47^phox and p67^phox transferred to the membrane of HUVECs. However， the content of SOD increased， NADPH and MDA decreased， and p40^phox， p47^phox and p67^phox were inhibited to transfer to the membrane， after the medication of BXRM serum. CONCLUSION BXRM serum can inhibited the oxidation of AngⅡ induced HUVEC damage and prevent arteriosclerosis.