Rapid Determination of Bornyl Acetate in Amomum Villosum by Near-Infrared Spectroscopy with Partial Least Square

OBJECTIVE To rapidly determine the content of bornyl acetate in amomum villosum by near-infrared spectroscopy (NIRS). METHODS The content of bornyl acetate in 101 amomum villosum was determined by GC. The quantitative analysis model of bornyl acetate in amomum villosum was established by collecting near-infrared spectra combined with partial least squares (PLS). RESULTS The correlation coefficients (R²) ， the root-mean-square error of calibration (RMSEC) and the root-mean-square error of cross-validation (RMSECV) of the quantitative calibration model of bornyl acetate were 0.992 59， 0.014 5 and 0.071 4; the root-mean-square error of prediction (RMSEP) was 0.016 7. CONCLUSION NIRS is simple and accurate， which can be used for rapid determination of bornyl acetate in amomum villosum.