Protective Effect of Ganoderic Acid A on Hepatic Injury Induced by D-GalN/LPS in Mice

OBJECTIVE To observe the effect of ganoderic acid A (GA) on D-GalN/LPS induced liver injury in mice. METHODS 60 male C57BL/6 mice were randomly divided into 4 groups: normal control group， D-GalN/LPS group， D-GalN/LPS+ GA (20 mg/kg) group， D-GalN/LPS+GA (40 mg/kg) group. Mice in the normal control group and D-GalN/LPS group were given distilled water while other groups were given drugs for 7 d by gavage. After the last dose of 4 h， D-GalN (700 mg/kg) and LPS (1 mg/kg) were given by intraperitoneal injection， and the mice in normal control group were given same volume of vegetable oil solution. 24 h after established model， the blood and liver were collected. Aspartate aminotransaminase（AST），alanine aminotransaminase（ALT），interleukin-6 (IL-6)， interleukin-1β (IL-1β) and tumor necrosis factor (TNF-α) were measured by corresponding kits. Hematoxylin (HE) staining was used to observe the changes of hepatic histopathology.Western blotting was used to demonstrate the expression levels of related protein. RESULTS GA significantly reduced levels of AST，ALT，IL-6， IL-1β and TNF-α in serum. In addition， GA regulated proteins levels of NLRP3/NF-κB pathway in liver. CONCLUSION GA shows the therapeutical effect on D-GalN/LPS induced liver injury in mice which may be related to the regulation of NLRP3/NF-κB pathway.