Volume 35 Issue 3
May  2019
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ZHANGJun-li, XIAOMin, WANGPeng, ZHANGXian, PANYang, YANGGuang-ming. The Inhibitory Effect and Mechanism of Neoliensinine on the Contraction of Mesenteric Vascular Smooth Muscle[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(3): 313-318.
Citation: ZHANGJun-li, XIAOMin, WANGPeng, ZHANGXian, PANYang, YANGGuang-ming. The Inhibitory Effect and Mechanism of Neoliensinine on the Contraction of Mesenteric Vascular Smooth Muscle[J]. Journal of Nanjing University of traditional Chinese Medicine, 2019, 35(3): 313-318.
shu

The Inhibitory Effect and Mechanism of Neoliensinine on the Contraction of Mesenteric Vascular Smooth Muscle

  • 1.

    School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China

  • 2.

    Laboratory of Medical Fungi and Phyto-Biotech, Nanjing University of Chinese Medicine, Nanjing, 210023, China

  • 3.

    Key Laboratory of State Administration of Traditional Chinese Medicine for Standardization of Chinese Medicine Processing, Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing, 210023, China

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    • Abstract
  • OBJECTIVE To observe the inhibition effect of neoliensinine (Neo) on the contraction of mesenteric vascular smooth muscle and vascular smooth muscle cells (VSMCs) of mice， and to explore the mechanism of the effect by observing phosphorylation of myosin light chain (MLC20) in vascular smooth muscle and expression of ROCK1 (RhoA associated protein kinase) in VSMCs. METHODS The microvascular tension tester was used to evaluate the inhibition effect of Neo pretreatment on mesenteric vessel contraction stimulated by high potassium solution， and Urea/glycerol -PAGE was applied to detect its change of phosphorylation of MLC20. Meanwhile， image analyzer was used to detect the effect of Neo on VSMCs contraction induced by high potassium solution， and cell immunofluorescence assay was adopted to detect its expression of ROCK1 protein in VSMCs. RESULTS Neo had a significant inhibitory effect on the contraction of mesenteric vessel induced by high potassium solution with IC50 of 6.019 μmol/L and 95% confidence intervals from 5.299～6.838 μmol/L， and could block the phosphorylation of MLC20 induced by high potassium. In addition， compared with the VSMCs length of model group (KCl) (40.94±1.94)μm， that of treatment group (Neo+KCl) were prolonged (44.95±5.15)μm significantly (P<0.01)， and Neo reduced the expression of ROCK1 protein in VSMCs. CONCLUSION Neo could inhibit the contraction of mesenteric vascular smooth muscle， and its mechanism might be related to blocking the phosphorylation of smooth muscle MLC20 and down-regulating the expression of ROCK1 protein in VSMCs.
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