Study on Mechanism of Pushen Capsule for Treating Hyperlipidemia Based on Network Pharmacology

OBJECTIVE Based on the network pharmacology technology， this research explores the medicinal substance basis and target of the Pushen capsule， and constructs a network of medicinal potential targets to explain the possible mechanism of the Pushen capsule in the treatment of hyperlipidemia， and provide research ideas for the pharmacodynamic basis of the Pushen capsule. METHODS Firstly， based on the Arrowsmith non-document prediction tool， the correlation between the Pushen capsule and its components and hyperlipidemia was discussed. Using the Chinese medicine ingredient database combined with the Percepta platform ADME/T predictive function to screen the active ingredients of the Pushen capsule， and determining the active ingredient target with the Similarity Ensemble Approach， STITCH and Benchmark Database molecular target sites. Applying GeneCards to search for hyperlipidemia-related disease targets， then combining active compound targets and identifying potential targets for drugs and hyperlipidemia， finally visualizing the results with cytoscape. Target annotation and classification can be made by using of the KEGG database and the DAVID database. RESULTS Through analyzing date of Arrowsmith， there was a significant correlation between the Pushen capsule and hyperlipidemia， as well as related diseases such as "atherosclerosis" and "diabetes"， suggest that Pushen capsule has therapeutic effects on these diseases. A total of 75 active ingredients were screened by pharmacological methods， and the main target was 261 and Critical targets associated with inflammatory responses. Based on the KEGG and DAVID databases， the effect of Pushen capsule on improving blood circulation and removing phlegm was mainly through the following signal pathways: ①AGE-RAGE signaling pathway，②Malaria related pathway，③hepatitis B related pathway，④VEGF signaling pathway，⑤Cancer related pathway. CONCLUSION The treatment of hyperlipidemia with Pushen Capsule is through multiple targets and multiple pathways. The main effort is focused on reducing chronic inflammation.