Simultaneously Determination of Eight Components in Schisandra chinensis Georgi by UPLC-MS/MS

OBJECTIVE To develop a sensitive and selective ultra performance liquid chromatography-triple quadrupole mass spectrometer (UPLC-MS/MS) method for quantification of eight components (chrysin， baicalein， apigenin， orohylin A， wogonin， baicalin， wogonoside， oroxin A) in Schisandra chinensis Georgi. METHODS The separation was performed on a Thermo BDS Hypersil C₁₈ (2.1mm×100mm， 2.3μm) column. The mobile consisted of acetonitrile (A) and 0.1% formic acid aqueous with a gradient elution at a flow-rate of 0.25mL/min. The detection was acquired by MRM. The column temperature was 40℃. RESULTS The linear ranges of chrysin， baicalein， apigenin， orohylin A， wogonin， baicalin， wogonoside， oroxin A were 0.975~62.5ng/mL (r=0.9998)， 4.875~2500ng/mL (r=0.9997)， 0.975~62.5ng/mL (r=0.9994)， 0.0975~12.5ng/mL (r=0.9996)， 0.04883~6.25ng/mL (r=0.9999)， 4.875~2500ng/mL (r=0.9998)， 0.4883~250ng/mL (r=0.9999)， 0.4883~250ng/mL(r=0.9998)， respectively. The recoveries were 98.75%，96.44%，101.13%，99.41%，102.26%，98.45%，96.75%，99.42%. CONCLUSION The proposed method enables quantification for the study of the compounds of chrysin， baicalein， apigenin， orohylin A， wogonin， baicalin， wogonoside， oroxin A in Schisandra chinensis Georgi