Effect of Bushen Huoxue Jiangu Decoction Drug Serum on Osteoblast Proliferation， Differentiation， Maturation and Mineralization in Vitro

OBJECTIVE To observe the effects of Bushen Huoxue Jiangu Decoction drug serum on osteoblast proliferation， differentiation， maturation and mineralization in vitro， which will provide the theoretical basis for osteoporosis treatment. METHODS Cancellous bone specimens from the femoral head were obtained from patients with femoral neck fracture merger osteoporosis， and the osteoblast cells were seeded and cultured in vitro to establish the osteoblast differentiation model. High， middle and low doses of Bushen Huoxue Jiangu Decoction drug serum were prepared. MTT method， ALP activity assay and alizarin red staining were used to observe the proliferation， activity of ALP and the number of mineral node of cultured osteoblasts in vitro. RESULTS MTT assay displayed different degrees of osteoblast proliferation in high and middle dose groups and showed some dose dependence after 48h of drug intervention. The activities of ALP in high， middle and low dose groups obviously increased after 72h of drug intervention. The deposition of calcium salt crystal in alizarin red staining obviously increased in high dose group after 72h of drug intervention. CONCLUSION Bushen Huoxue Jiangu Decoction drug serum can promote osteoblast proliferation， differentiation， maturation and mineralization in vitro.