Determination of Astragaloside Ⅳ and Calycosin-7-O-β-D-glucopyranoside in Qishen Oral Liquid by HPLC

OBJECTIVE To develop a HPLC method for the determination of astragaloside Ⅳ and calycosin-7-O-β-D-glucopyranoside in Qishen oral liquid. METHODS The astragaloside Ⅳ was determinated by ELSD with C18 column. Acetonitrile-water (ratio-32∶68) was set as the mobile phase， the temperature of drift tube was 102 ℃ and the gas flow rate was 2.7 L/min. DAD was used to detect calycosin-7-O-β-D-glucopyranoside， and the mobile phase was composed of acetonitrile and 0.2% formic acid aqueous for gradient elution with the detecting wave length of 260 nm. RESULTS The linear ranges of astragaloside Ⅳ and calycosin-7-O-β-D-glucopyranoside were 1.1～16.5 μg and 0.038～0.382 μg respectively. The average recoveries of two ingredients were 96.73% (RSD=2.08%) and 100.70% (RSD=3.12%). CONCLUSION The analytic results determined by HPLC were accurate， which can be used as reference for quality control in astragalus preparation.