Effects of 5-Fu on Mice with Spleen Deficiency Syndrome

OBJECTIVE To study the effects of 5-Fu on mice with spleen deficiency syndrome and the content of short chain fatty acid in intestine. METHODS After the injection of 5-Fu in abdomen， data such as food intake， body weight changes， diarrhea and other information were collected to observe symptoms due to spleen deficiency. Endo-intestinal toxicity of 5-Fu were evaluated by HE staining and detecting beta-D-galactose glucoside enzyme activity in intestinal mucosa at the same time. The content of short chain fatty acids in intestinal contents were also determined to investigate the influences of 5-Fu on the intestinal flora. RESULTS Compared with control group， the spleen deficiency syndrome may induced by 5-Fu， the mice appeared the symptoms， such as poor appetite， weight loss and diarrhea， and it was shown in the physiological section that the intestine villis significantly shortened， the pathological changes further developed， the contents of TNF-α and IL-6 in plasma increased and the beta-D-galactose glucoside enzyme activity of intestinal mucosa decreased. Contents of acetic acid， butyric acid and pentanoic acid in intestine significantly reduced at the same time. CONCLUSION Fu may induce spleen deficiency syndrome in mice and significantly damage the intestinal mucosa and intestinal flora， which indicate that when 5-Fu is applied， some spleen-strengthening medicine may be used to reduce induced intestinal side effects.