Biosynthesis of Puerarin Glycoside and Proliferation Inhibition Effect on Breast Cancer Cell

OBJECTIVE To study the optimal transforming conditions for biosynthesis of puerarin glycoside by Acinetobacter johnsonii G2， and the proliferation inhibition effect of puerarin glycoside on human breast cancer cell line MDA-MB-231. METHODS Effect of temperature， pH and sucrose concentration on transformation rate in biosynthesis system were studied. Besides， effects of different concentrations of puerarin and puerarin glycoside on the proliferation of MDA-MB-231 cells through methyl thiazolyl tetrazolium trace enzyme reaction colorimetry (MTT assay) were investigated. RESULTS The best transformation condition of puerarin glycoside was 30 ℃， pH 7.38 and sucrose concentration of 60 g/L. In this condition， the transformation rate was 85% after 24 h. MTT assays showed that different concentrations of puerarin and puerarin glycoside all could inhibit proliferation of MDA-MB-231 cells， and the inhibition effect strengthened with the concentration increased; among which the inhibition rate of 200μmol/L puerarin and puerarin glycoside on MDA-MB-231 cells were 61.91% and 59.42% respectively after 48 h. CONCLUSION Acinetobacter johnsonii G2 can effectively catalyze synthesis of puerarin to puerarin glycoside; both puerarin and puerarin glycoside can significantly inhibit the proliferation of human breast cancer cell line MDA-MB-231.