Study on Quality Standard of Wuwei Hugan Chewable Tablets

OBJECTIVE To establish quality standard of Wuwei Hugan Chewable Tablets.METHODS With chisantherin B， Deoxyschisandrin and Schisandra as control materials， Wuwei Hugan Chewable Tablet is identified by thin layer chromatography (TLC); the content of schisandrin， schisantherin B， schisantherin A， Deoxyschisandrin and Schisandrin B， are simultaneously determinated by HPLC.RESULTS Excellent chromatographic separation was achieved in the five tested lignans with good linearity (r>0.999 5) over the studied concentration ranges. The average extraction recovery (RSD)(n=9) was 99.90%(1.63%) for Schisandrin， 99.42%(1.72%) for Schisandrol B， 99.81%(2.07%) for Schisantherin A， 99.79%(1.91%) for Deoxyschisandrin and 100.57%(1.61%) for Schisandrin B.CONCLUSION Specific， sensitive and reliable TLC identification and HPLC content determination of Wuwei Hugan Chewable Tablets were established. The TLC and HPLC methods developed for simultaneous determination of five lignans are simple and valid. It can be used for comprehensively controlling quality of Wuwei Hugan Chewable Tablets.