Preliminary Evaluation and Preparation of Brucine Nanoparticles-in-microsphere System

OBJECTIVE To find a long-acting preparation which can reduce the burst effect in intra-articular injection by preparing the brucine nanoparticles-in-microsphere system (Brucine-NiMS).METHODS Brucine nanoparticles (Brucine-Nps) were prepared by desolvation of BSA. Chitosan was used as an carrier and the nanoparticles were contained in the microsphere system by the spray drying method. The appearances of Brucine-NiMS were observed under the electron microscope and then the in vitro release characteristics were determined.RESULTS The formulation of Brucine-NiMS was as follows: BSA/CS (600KDA) 1∶5 (w/w) and the concentration of CS in the acetic acid was 1%. The spry drying conditions were as follows: The speed of the peristaltic pump was adjusted to 20%， the temperature of inlet was 120 ℃， and Q-Flow was 40 mL/h. The envelop rate， drug loading and particle sizes of Brucine-NiMS were 97.35%， 3.17% and 1.48 μm respectively. The appearance of Brucine-NiMS was rounding and the nature was stable.CONCLUSION The preparation method of Brucine-NiMS is stable and controllable， providing a new way to develop new dosage forms of drugs.