The Effect of Hederagenin on the Proliferation， Adhesion， Invasion and Migration of Human Colon Cancer Cells LoVo

OBJECTIVE To explore the effect of hederagenin on the proliferation， adhesion， invasion and migration of human colon cancer cell line LoVo in vitro.METHODS MTT assay， adhesion experiment， Transwell room invasion test， and erasion trace test were used to observe the effect of hederagenin on the proliferation， adhesion， invasion and migration.RESULTS ①Hederagnin inhibited the proliferation of LoVo cells. Compared with control group， the inhibition ratio had been significantly increasing with the increase of the drug concentration， which showed a concentration dependence (P<0.01). ②Hederagnin remarkably reduced the adhesion capability of LoVo cells (P<0.01)， and along with the increasing drug concentration and time， the inhibition ratio increased. ③Compared with control group， the number of invaded cells was significantly decreased (P<0. 01). The higher the drug concentration was， the less cells were invaded. ④The cell healing degree had an obvious group difference， and the higher the drug concentration was， the worse the healing degree was， which indicated that migration ability was greatly affected.CONCLUSION Hederagenin can remarkably inhibit the proliferation， adhesion， invasion and migration of LoVo cells.