Effects of Qingtouxiere Decoction on Expression of TLR-7， MyD88 and NF-κB mRNA and Protein in Ana-1 Cells of Influenza A H1N1 Influenza Virus

OBJECTIVE To observe the effect mechanism of Qingtouxiere Decoction on anti-influenza A H1N1 influenza virus in vitro. METHODS The macrophages were stimulated with FM1 in the lungs of influenza A virus， respectively， with different concentrations of 0.388mg/mL， 0.194mg/mL， 0.097mg/mL， 0.0485mg/mL and 0.02425mg/mL. The expression of TNF-α， IP-10 and IL-6， TLR-7， mRNA and protein expression of MyD88 and NF-κB. RESULTS The expression of TNF-α， IP-10 and IL-6 in macrophages of Ana-1 mice was inhibited by Qingtouxiere Decoction， and the infection of H1N1 virus was decreased in 0.02425mg/mL group (P<0.01). The level of TNF-α was significantly lower in the group of Qingtouxiere Decoction 0.097mg/mL (P <0.01). The expression of TLR-7， MyD88 and NF-κB mRNA and protein were increased after Ana-1 macrophage infection in H1N1 influenza virus group. The expression of TLR-7， MyD88 and NF-κB mRNA was increased by 0.0485mg/mL group (P<0.01). The expression of MyD88 mRNA in the H1N1 infected group was significantly higher than that in the control group (P <0.01). The expression of TLR-7 mRNA was significantly inhibited The expression of NF-κB mRNA in H1N1 virus group was significantly higher than that in H1N1 virus group (P<0.01). The expression of TLR-7 protein in H1N1 virus group was significantly higher than that in H1N1 virus group(P<0.01). The expression of NF-κB in the H1N1 infected group was significantly higher than that in the H1N1 virus group (P<0.01). The expression of NF-κB in the H1N1 infected group was significantly higher than that in the control group (P<0.01). CONCLUSION The activation of TLR-7/MyD88/NF-κB signaling pathway induced by H1N1 influenza virus can inhibit the expression of downstream inflammatory factors and play the antiviral effect.