The Anti-hyperglycemic Activity of Crude Polysaccharides from Mactra Veneriformis in Alloxan Induced Diabetes in Mice

OBJECTIVE To study the anti-hyperglycemic effective sites of crude polysaccharides from Mactra veneriformis (MVPS). METHODS  Hyperglycemia models were established by intravenous injection of alloxan via tail vein of ICR mice under a dose of 80 mg/kg. After 96 h， mice with fasting blood glucose levels≥11.1 mmol/L were chosen and divided into groups. MVPS 1 treated at the doses of 200， 400， 800 mg/kg and 1 200 mg/kg once daily， respectively. At 7th and 14th days， respectively， the levels of blood glucose， GSP， TCH and TG were measured. Other diabetic mice were divided into MVPS 1 (800 mg/kg)， MVPS 2 (180， 360 mg/kg) and MVPS 3 (2 040 mg/kg) groups. After 7 d treatment， both body weights and fasting blood glucose levels were measured. RESULTS MVPS 1 of 800 mg/kg displayed the optimal anti-hyperglycemic effect on diabetic mice and reduced GSP， TCH and TG levels of diabetic mice; MVPS 2 showed significantly anti-hyperglycemic activities， compared to the model group. MVPS 3 exhibited no anti-hyperglycemic activities. CONCLUSION MVPS 1 displays the optimal hypoglycemic effect at the dose of 800 mg/kg， and its hypoglycemic active site focuses on MVPS 2.