OBJECTIVE To analyze the plasma-entering components of the ethanol extract of Yu-Ping-Feng-San by ultra-performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UPLC-Q-TOF-MS) technology, so as to provide a reference for the study of the pharmacological substances of Yu-Ping-Feng-San.
METHODS Male BALB/c mice were gavaged with the ethanol extract of Yu-Ping-Feng-San (6.5 g ·kg-1), and plasma samples were collected before administration (0 h) and 1, 2, 4, 6, 8, and 12 h after administration. The UPLC-Q-TOF-MS technology was used to analyze and identify the components absorbed into the plasma.
RESULTS A total of 73 chemical components in the ethanol extract of Yu-Ping-Feng-San were identified, and 26 plasma-entering components were identified in all plasma samples. Among the plasma-entering components, 11 components were from Saposhnikoviae Radix, and 15 components were from Radix Astragali.
CONCLUSION The chemical components and plasma-entering components of the ethanol extract of Yu-Ping-Feng-San were analyzed comprehensively, which provides a reference for further clarifying the pharmacological substance basis and mechanism of action of Yu-Ping-Feng-San.
DownLoad: