OBJECTIVE To establish a extraction process of Changyan Heji Ⅱ (CYHJ-Ⅱ) based on UPLC-Q-TOF-MS/MS technology combined with response surface analysis, and to optimize the extraction process.
METHODS The chemical components in CYHJ-Ⅱ were qualitatively analyzed by UPLC-Q-TOF-MS/MS technology, and the chemical components with good linear relationship in mass spectrometry response were selected as process investigation indicators; the extraction process parameters (water addition amount, extraction time and soaking time) were investigated by Box-Behnken design; the comprehensive score was obtained by principal component analysis (PCA), and the optimal process was determined by the comprehensive score combined with response surface analysis.
RESULTS Through qualitative analysis, 110 components were inferred and identified from CYHJ-Ⅱ, including 2 organic acids, 82 flavonoids, 13 terpenoids, and 13 alkaloids. Based on the results of qualitative analysis, 48 index components with good linear relationships were derived by UPLC-Q-TOF-MS/MS combined with Masshunter mass spectrometry data analysis software. PCA was performed and the comprehensive score was calculated. Response surface analysis was performed with the comprehensive score as an indicator. The optimal extraction process obtained by combining the response surface prediction results and actual production was: soaking for 45 min, 8 times the amount of solvent, 2 extractions, each time for 120 min.
CONCLUSION This study provides a new idea for the investigation of the extraction process of traditional Chinese medicine compound prescriptions and expands a new method for the development of traditional Chinese medicine compound prescriptions.
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