UPLC-Q-TOF-MS Analysis on Chemical Constituents of Classical Prescription Xiehuang San Standard Decoction

  OBJECTIVE   To analyze the chemical constituents from classical prescription Xiehuang San(XHS) standard decoction by UPLC-Q-TOF-MS technology, and classify the chemical composition and analyze the representative components.

  METHODS   Acquity HSS T₃ column (2.1 mm × 100 mm, 1.8 μm) was used as the chromatographic column, with 0.1% formic acid solution-0.1% formic acid acetonitrile as the mobile phase for gradient elution. The volume flow rate was 0.4 mL·min^-1 and the column temperature was 40 ℃. Mass spectrometry data of XHS were collected in positive and negative ion modes. The chemical constituents from classical prescription XHS were analyzed and identified by Masslynx 4.1 software comparison with reference materials, mass spectrometry data analysis and reference to relevant literature.

  RESULTS   A total of 107 compounds were analyzed and identified from XHS, including 45 flavonoids, 27 triterpenoids, 11 monoterpenoids, 10 phenylpropanoids, 6 chromogenic ketones, 5 alkaloids and 3 other other compounds.

  CONCLUSION   The study provides an experimental basis for the further research on the substance basis and quality control of XHS.