OBJECTIVE To study the structural characteristics of purified polysaccharide isolated from Polygonatum cyrtonema Hua and explore its anti-fatigue and intestinal bacteria improvement effects, so as to provide theoretical basis for the development of products using this polysaccharide as functional ingredients.
METHODS PMP pre-column derivatization-high performance liquid chromatography, trimethylsilyl ether derivatization-gas chromatography and other methods were used to chemically characterize the purified polysaccharide; the mouse weight-loading swimming test and biochemical indicators were used to evaluate its anti-fatigue effect; 16S rRNA intestinal flora sequencing was adopted to study the effect of the purified product in improving intestinal bacteria.
RESULTS The obtained polysaccharide was a fructan product and contained small amounts of glucose (Glc), galactose (Gal), arabinose (Ara), mannose (Man), rhamnose (Rha) and galacturonic acid (GalA), which had a relative molecular mass in the range of 0.4-285 kDa, and the molecular weight of the main component was around 3 kDa. Compared with the model group, the high-dose polysaccharide group could significantly prolong the exhaustive swimming time of mice (P < 0.01), reduce the levels of BUN and UA in serum and the LA (P < 0.01) and MDA levels (P < 0.05) in muscle tissue, and enhance SOD (P < 0.01), GSH-Px (P < 0.001) and CAT (P < 0.05) levels in liver tissue. Compared with the blank control group, the low-dose polysaccharide group could increase the relative abundance of probiotics Eggerthelaceae (g_unclassified_f_Eggerthelaceae) and Weissella (g_Weissella) in the fecal microorganisms of mice (P < 0.05).
CONCLUSION The chemical composition of purified polysaccharide isolated from Polygonatum cyrtonema Hua is determined, and it is proven that the polysaccharide has a good anti-fatigue effect and can promote the growth of probiotics in the intestine, which provides a basis for product development using this polysaccharide as a functional ingredient.
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