OBJECTIVE To investigate the therapeutic effect of Huashi Baidu Granules on respiratory syncytial virus (RSV) pneumonia model mice and its effect on lung lipid metabolism.
METHODS BALB/c mice were randomly divided into control group, model group, Ribavirin group, Dexamethasone group, low dose group of Huashi Baidu Granules and high dose group of Huashi Baidu Granules. Lung tissue samples of mice in each group were collected to observe pathological changes and detect mRNA expression levels of RSV-F, RSV-G, IL-1β, IL-6 and TNF-α genes. The lipid components of lung tissue were extracted and analyzed by ultra-high performance liquid chromatography-quadrupole-electrostatic field orbital trap high-resolution mass spectrometry (UPLC-Q Exactive Orbitrap MS) to find the changes in lipid metabolism.
RESULTS The model group exhibited pulmonary interstitial thickening and inflammatory infiltration compared to the control group, the mRNA levels of viral load and inflammatory factors in lung tissue such as IL-1β and TNF-α were significantly increased (P < 0.001). Moreover, the model group exhibited significant abnormalities in lipid metabolism, including disorders in phosphatidylserine (PS), phosphatidylglycerol (PG), sphingomyelin (SM), ceramide (Cer), triglyceride (TG), diglyceride (DG), fatty acid (FA), ether phosphatidylcholine (PC O), ether lysophosphatidylcholine (LPC O). However, after the treatment of Huashi Baidu Granules (25.12 g·kg-1·d-1), the above indexes showed a significant improvement.
CONCLUSION Huashi Baidu Granules can treat RSV pneumonia by regulating lipid metabolism, reducing viral load and alleviating inflammatory response in lung tissue.
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