Volume 40 Issue 3
Mar.  2024
Turn off MathJax
Article Contents
Abstract
References
LIU Lu, YU Hongli, WU Hao, WANG Xinzhi, CUI Xiaobing, CAO Yu, LIN Weihao, SHEN Changli, CAO Jie. Changes in Intestinal Toxicity and Component Analysis of Mongolian Medicine Euphorbia Pekinensis Rupr before and after Processing with Milk[J]. Journal of Nanjing University of traditional Chinese Medicine, 2024, 40(3): 278-288. DOI: 10.14148/j.issn.1672-0482.2024.0278
Citation: LIU Lu, YU Hongli, WU Hao, WANG Xinzhi, CUI Xiaobing, CAO Yu, LIN Weihao, SHEN Changli, CAO Jie. Changes in Intestinal Toxicity and Component Analysis of Mongolian Medicine Euphorbia Pekinensis Rupr before and after Processing with Milk[J]. Journal of Nanjing University of traditional Chinese Medicine, 2024, 40(3): 278-288. DOI: 10.14148/j.issn.1672-0482.2024.0278
shu

Changes in Intestinal Toxicity and Component Analysis of Mongolian Medicine Euphorbia Pekinensis Rupr before and after Processing with Milk

  • 1.

    School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China

  • 2.

    Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing 210023, China

  • 3.

    Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210023, China

  • 4.

    Nanjing Institute for Food and Drug Control, Nanjing 211198, China

More Information
  • Received Date: October 24, 2023
  • Available Online: March 14, 2024
    Graphical Abstract
    • Abstract
  •   OBJECTIVE  To explore the correlation between changes in intestinal toxicity and changes in component composition of the Mongolian medicine Euphorbia pekinensis Rupr (EPR) before and after processing with milk.
      METHODS  Mice were given 95% ethanol extract of raw EPR, milk-processed EPR and water-processed EPR by gavage. The purgative effect and intestinal inflammatory toxicity changes of EPR before and after milk processing were investigated using the fecal water content and the levels of inflammatory factors TNF-α and IL-1β in each intestinal segment of mice as indicators; LC-MS/MS was used to analyze the composition changes of the 95% alcohol extract of EPR before and after milk processing.
      RESULTS  Compared with the blank group, the raw and water processed products of EPR could significantly increase the water content of mouse feces and the levels of TNF-α and IL-1β in each intestinal segment (P < 0.05); compared with the raw product group, all indicators in the milk processing group were significantly reduced (P < 0.05), while there was no significant difference in the water processing group, indicating that water processing cannot attenuate toxicity, and the auxiliary material milk is the key auxiliary material to reduce the toxicity of EPR. Mass spectrometry analysis results showed that a total of 50 components were identified in EPR, including 38 terpenoid components, 6 phenolic acid components, and 6 other components. The content of each component decreased to varying degrees after milk processing. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed on the mass spectrum data of raw materials and products, and it was found that the components of raw materials and products can be obviously clustered into 2 categories. 13 differential components of raw materials and products were screened through t test, and 11 of which were terpene components, indicating that the composition of terpene components changed significantly after milk processing. 17 components derived from EPR were detected in the residual liquid of milk excipients after processing, of which 16 were terpenoids, indicating that the terpenoid components of EPR were transferred to the excipient milk during the soaking and processing processes.
      CONCLUSION  The toxicity of EPR is reduced and the purgative effect is alleviated after milk processing. The attenuation mechanism may be that during the milk soaking and processing processes, terpenoid components are transferred to the milk, and the content of toxic components in the decoction pieces is reduced, thereby reducing the toxicity.
    • FullText(HTML)
    • References (18)
  • [1]
    国家药典委员会. 中华人民共和国药典: 一部[M]. 北京: 中国医药科技出版社, 2020: 89-90.

    Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China: Part Ⅰ[M]. Beijing: China Medical Science Press, 2020: 89-90.
    [2]
    WANG K L, XU X F, MAIMAITI A, et al. Gut microbiota disorder caused by diterpenoids extracted from Euphorbia pekinensis aggravates intestinal mucosal damage[J]. Pharmacol Res Perspect, 2021, 9(5): e00765. DOI: 10.1002/prp2.765
    [3]
    田华咏. 中国民族药炮制集成[M]. 北京: 中医古籍出版社, 2000.

    TIAN H Y. Integration of ethnic medicine processing in China[M]. Beijing: Ancient Chinese Medical Book Press, 2000.
    [4]
    内蒙古自治区药品监督管理局. 内蒙古蒙药饮片炮制规范: 2020年版[M]. 呼和浩特: 内蒙古人民出版社, 2020.

    Drug Administration of Inner Mongolia Autonomous Region. Processing standard of Mongolian medicine pieces in Inner Mongolia: 2020 edition[M]. Hohhot: Inner Mongolian People's Publishing House, 2020.
    [5]
    宇妥·元丹贡布. 四部医典[M]. 马世林, 译注. 上海: 上海科学技术出版社, 1987.

    Yuthog Yonten Gonpo. Medical Canon in Four Sections[M]. MA S L, translated. Shanghai: Shanghai Scientific & Technical Publishers, 1987.
    [6]
    WANG K L, LIU L, HUANG J Y, et al. Laxative effects of total diterpenoids extracted from the roots of Euphorbia pekinensis are attributable to alterations of aquaporins in the colon[J]. Molecules, 2017, 22(3): 465. DOI: 10.3390/molecules22030465
    [7]
    WANG K L, YU H L, WU H, et al. A new casbane diterpene from Euphorbia pekinensis[J]. Nat Prod Res, 2015, 29(15): 1456-1460. DOI: 10.1080/14786419.2015.1027704
    [8]
    张元斌. 大戟属有毒中药狼毒和京大戟醋制解毒存效机制研究[D]. 南京: 南京中医药大学, 2021.

    ZHANG Y B. Study on the mechanism of reducing toxicity and preserving effect of Euphorbiae Ebracteolatae radix and Euphorbiae Pekinensis radix processing by vinegar[D]. Nanjing: Nanjing University of Chinese Medicine, 2021.
    [9]
    徐晓芬, 郁红礼, 吴皓, 等. 京大戟醋制改善肠道菌群紊乱降低肠毒性作用研究[J]. 中国中药杂志, 2022, 47(5): 1243-1252. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY202205015.htm

    XU X F, YU H L, WU H, et al. Vinegar-processed Euphorbiae Pekinensis Radix improves intestinal flora disorder and reduces colon toxicity[J]. China J Chin Mater Med, 2022, 47(5): 1243-1252. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY202205015.htm
    [10]
    姚芳, 张楷承, 曹雨诞, 等. 京大戟醋制前后对斑马鱼胚胎肝、胃肠毒性的影响[J]. 中国中药杂志, 2019, 44(6): 1179-1185. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201906017.htm

    YAO F, ZHANG K C, CAO Y D, et al. Effects of Euphorbiae Pekinensis Radix before and after processing with vinegar on liver and gastrointestinal toxicity of zebrafish embryos[J]. China J Chin Mater Med, 2019, 44(6): 1179-1185. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201906017.htm
    [11]
    YAO F, JIANG D J, CHENG F F, et al. Diterpene pekinenal from Euphorbia pekinensis radix induced IEC-6 cells apoptosis mediated by mitochondria and death receptors[J]. Toxicol Vitro, 2019, 57: 1-8. DOI: 10.1016/j.tiv.2019.01.019
    [12]
    王奎龙, 郁红礼, 吴皓, 等. 京大戟毒性部位及其醋制前后成分变化研究[J]. 中国中药杂志, 2015, 40(23): 4603-4608. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201523013.htm

    WANG K L, YU H L, WU H, et al. Totoxicity fraction from Euphorbia pekinensis and composition change after vinegar processing[J]. China J Chin Mater Med, 2015, 40(23): 4603-4608. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201523013.htm
    [13]
    殷文志, 王婷钰, 朱拓, 等. 三种品牌牛奶的荧光光谱特性研究[J]. 光谱学与光谱分析, 2021, 41(2): 535-539. https://www.cnki.com.cn/Article/CJFDTOTAL-GUAN202102038.htm

    YIN W Z, WANG T Y, ZHU T, et al. The study on fluorescence spectral characteristics of three brands of milk[J]. Spectrosc Spectr Anal, 2021, 41(2): 535-539. https://www.cnki.com.cn/Article/CJFDTOTAL-GUAN202102038.htm
    [14]
    GLAB T K, BORATYNSKI J. Potential of casein as a carrier for biologically active agents[J]. Top Curr Chem, 2017, 375(4): 71. DOI: 10.1007/s41061-017-0158-z
    [15]
    TIAN X H, WANG P L, LI T, et al. Self-assembled natural phytochemicals for synergistically antibacterial application from the enlightenment of traditional Chinese medicine combination[J]. Acta Pharm Sin B, 2020, 10(9): 1784-1795. DOI: 10.1016/j.apsb.2019.12.014
    [16]
    TRAN N P, TRAN P, YOO S Y, et al. Sialic acid-decorated liposomes enhance the anti-cancer efficacy of docetaxel in tumor-associated macrophages[J]. Biomater Adv, 2023, 154: 213606. DOI: 10.1016/j.bioadv.2023.213606
    [17]
    ALLAHDAD Z, VARIDI M, ZADMARD R, et al. Spectroscopic and docking studies on the interaction between caseins and β-carotene[J]. Food Chem, 2018, 255: 187-196. DOI: 10.1016/j.foodchem.2018.01.143
    [18]
    SAIZ-ABAJO M J, GONZALEZ-FERRERO C, MORENO-RUIZ A, et al. Thermal protection of β-carotene in re-assembled casein micelles during different processing technologies applied in food industry[J]. Food Chem, 2013, 138(2/3): 1581-1587.
    • Related Articles

  • Cited by

    Periodical cited type(6)

    1. 程迎迎，刘志琴，郑凯，张洁，徐希. 清肠化湿方调控Nrf2/ARE通路缓解溃疡性结肠炎的机制研究. 南京师大学报(自然科学版). 2024(04): 86-97 .
    2. 房子铭，孙志文，谢晶日. 基于肠道菌群探讨中药治疗溃疡性结肠炎的研究进展. 中国医药导报. 2023(06): 42-45+58 .
    3. 王金鑫，谢胜，黄茂光，罗峰，陈月莹，黎丽群. 肠紧密连接在溃疡性结肠炎中的作用及中药治疗进展. 中国实验方剂学杂志. 2023(15): 249-255 .
    4. 柳沛林，周军，魏海梁，肖刚，郭辉，张迪. 中药调控Th17/Treg平衡治疗溃疡性结肠炎的研究进展. 中药新药与临床药理. 2023(11): 1632-1639 .
    5. 李光美，赵伟，张婷. 从肠道有益菌群可激活AhR/ILC3s信号通路探讨解毒化浊法改善2型糖尿病胰岛素抵抗的理论机制. 环球中医药. 2022(08): 1415-1417 .
    6. 徐文轩，张天鹏，任新荣，张瑞，韩福谦，吴吉海. 混合痔术后患者血清CXCR4、IL-8、IL-22水平与创面愈合及疼痛程度的相关性. 临床误诊误治. 2022(11): 69-72 .

    Other cited types(9)

Catalog

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (162) PDF downloads (21) Cited by(15)
    Related

    Copyright © Journal of Nanjing University of traditional Chinese Medicine

    Sponsored by：Nanjing University of traditional Chinese MedicineAddress：No.138 xianlin Avenue, Qixia District, Nanjing cityChina Pos：210023

    Tel：025-85811935Email：xb@njucm.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return