Study on the Effect of Moslae Herba Oil on the Percutaneous Absorption of Active Ingredient of Evodia Rutaecarpa in vitro

  OBJECTIVE  To investigate whether Moslae herba oil has penetration enhancing effect on the active components of Evodia rutaecarpa and its ability to promote transdermal absorption.

  METHODS  In vitro transdermal experiments were performed using the diffusion pool and the isolated skin of rats. Using various components of Evodia rutaecarpa as indicators, HPLC was determined to calculate the umulative penetration amount of each component. Changes in the skin and cuticle layers were observed by HE staining, immunohistochemical staining, and ATR-FTIR. GC-MS was used to measure Moslae herba oil components after administration.

  RESULTS  Moslae herba oil showed the penetration-enhancing effect on various components in Evodia rutaecarpa. Compared with the postive drug nitrogenone, the cumulative penetration amounts of hyperoside, evodiamine and rutaecarpine were greater. In addition, the sesquiterpene composition and thymol in the Moslae herba oil were more likely to be stored in the skin, which could disturb the lipid structure of the cuticle layer and promote the penetration of drugs.

  CONCLUSION  The results provide reference for the selection of transdermal absorption agent.