OBJECTIVE To screen the active components of Sanfeng Tongqiao (SFTQ) by using network pharmacological method and to investigate the target and mechanism of SFTQ in the treatment of allergic rhinitis (AR).
METHODS The active components and targets of SFTQ were screened by TCMSP. TCMSP and TTD database were used to screen AR-related therapeutic targets. SFTQ active components-AR target network was constructed by Cytoscape to predict potential targets. KEGG pathway enrichment analysis was performed on Davidv 6.8 database. An AR mouse model was established, and different doses of SFTQ were used for intervention during the stimulation period. The results were evaluated through behavioral indicators, pathological sections, HE staining, peripheral blood EOS and IgE levels, and the number of ILC2 in cervical lymph nodes.
RESULTS 42 active components and 16 potential targets were identified. KEGG enrichment analysis revealed 18 potential signaling pathways involving T cell receptor signaling pathway, HIF-1 signaling pathway, etc. In vivo, SFTQ significantly reduced the frequency of sneezing and rubbing, the degree of mucosal thickening, peripheral blood EOS, IgE level and the amount of ILC2 in cervical lymph nodes.
CONCLUSION Using systems pharmacology methods, the study revealed the multi-component, multi-target, and multi-pathway characteristics of SFTQ in treating AR, providing possible targets and active components for the mechanism study of SFTQ, and laying the foundation for a more comprehensive understanding and application of this prescription.
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