Study on Quality Evaluation of Jingqishen Oral Liquid Based on HPLC-Q-TOF-MS, Fingerprint and Content Determination

  OBJECTIVE  To establish HPLC-ELSD fingerprint of Jingqishen (JQS) oral liquid and the content determination method of its active ingredients.

  METHODS  An Agilent TC-C₁₈ (250 mm×4.6 mm, 5 μm) chromatographic column was used, and acetonitrile (A)-water solution (B) was adopted as the mobile phase for gradient elution to establish the HPLC-ELSD fingerprint of JQS oral liquid. The chemical composition of JQS oral liquid was analyzed by HPLC-Q-TOF-MS technology. The similarity evaluation was applied to study the data.

  RESULTS  A total of 75 compounds were identified, and 14 common peaks were calibrated by the fingerprint of 10 samples. Through comparison with reference standards, a total of 8 chromatographic peaks were identified: Spinosin, Jujuboside A, Ginsenoside Rg₁, Ginsenoside Re, Ginsenoside Rb₁, Ginsenoside Rd, Ginsenoside Rf, Ginsenoside Rg₃ were identified. In order to further control the overall quality of oral liquid, 4 active ingredients, Spinosin, Ginsenoside Rg₁, Ginsenoside Re and Ginsenoside Rb₁, were selected for content determination. Each active ingredient had a good linear relationship, and the established method had high sensitivity, good stability and accuracy.

  CONCLUSION  The methods of content determination and fingerprint measurement established in this study are accurate and stable, and they control the quality of the JQS oral liquid comprehensively.