Effects of Four Commonly Used Chinese Medicines with Clearing Heat and Drying Dampness on Intestinal Flora Mediated Bile Acids and Short Chain Fatty Acids Metabolism

LI Xin-ru; SHENG Xian-jie; YANG Yan; AN Zhen-tao; KANG An; LI Hui; GE Fei

doi:10.14148/j.issn.1672-0482.2023.0442

Volume 39 Issue 5

May 2023

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Journal of Nanjing University of traditional Chinese Medicine > 2023 > 39(5): 442-451. > DOI: 10.14148/j.issn.1672-0482.2023.0442

LI Xin-ru, SHENG Xian-jie, YANG Yan, AN Zhen-tao, KANG An, LI Hui, GE Fei. Effects of Four Commonly Used Chinese Medicines with Clearing Heat and Drying Dampness on Intestinal Flora Mediated Bile Acids and Short Chain Fatty Acids Metabolism[J]. Journal of Nanjing University of traditional Chinese Medicine, 2023, 39(5): 442-451. DOI: 10.14148/j.issn.1672-0482.2023.0442

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Effects of Four Commonly Used Chinese Medicines with Clearing Heat and Drying Dampness on Intestinal Flora Mediated Bile Acids and Short Chain Fatty Acids Metabolism

LI Xin-ru^{1, 2,},
SHENG Xian-jie^{1, 2},
YANG Yan¹,
AN Zhen-tao³,
KANG An^{1, 2},
LI Hui^3, ,,
GE Fei^1, ,

1.
Hai'an Hospital of Traditional Chinese Medicine Affiliated to Hanlin College of Nanjing University of Chinese Medicine, Nantong 226600, China
2.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
3.
Integrated Chinese and Western Medicine Hospital Affiliated to Nanjing University of Chinese Medicine, Jiangsu Academy of Traditional Chinese Medicine, Nanjing 210028, China

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Received Date: January 05, 2023
Available Online: May 18, 2023

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Abstract

Abstract

OBJECTIVE To explore the effects of four kinds of heat-clearing and dampness-drying herbs on intestinal flora, bile acids and short chain fatty acids (SCFAs) in normal mice at long-term clinical equivalent doses.
METHODS Thirty Balb/c male mice were randomly divided into control group, Sophora flavescens Ait. (KS) group, Phellodendron chinense Schneid. (HB) group, Coptis chinensis Franch. (HL) group and Scutellaria baicalensis Georgi (HQ) group, with 6 mice in each group. Except for the control group, KS group, HB group, HL group and HQ group were orally administrated at a dosage of 2.34 g·kg^-1, 3.12 g·kg^-1, 1.3 g·kg^-1 and 2.6 g·kg^-1, respectively, for two weeks. The contents of short chain fatty acids and bile acids in feces were detected by liquid chromatography tandem mass spectrometry, and the structural changes of microflora in intestinal contents were detected by 16S rRNA high-throughput gene sequencing technology.
RESULTS There was no significant effect of heat-clearing and dampness-drying Chinese medicine on the contents of six short-chain fatty acids in the feces of normal mice. Among the 23 common bile acids, when compared with the control group, the numbers of the significantly changed bile acids in HQ group, KS group, HL group and HB group were 4, 3, 2 and 1, respectively. From the perspective of changes in the abundance of intestinal flora, compared with the control group, except for the KS group, Firmicutes in the HL group, HQ group and HB group all showed a downward trend, and the Bacteroides showed an upward trend, among which the changes in the HL group were the most significant.
CONCLUSION Sophora flavescens Ait., Phellodendron chinense Schneid., Coptis chinensis Franch. and Scutellaria baicalensis Georgi have little effect on SCFAs in normal mice, but can affect bile acid content by changing the structure of intestinal flora.
- clearing heat and drying dampness,
- short-chain fatty acids,
- bile acids,
- intestinal flora

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Effects of Four Commonly Used Chinese Medicines with Clearing Heat and Drying Dampness on Intestinal Flora Mediated Bile Acids and Short Chain Fatty Acids Metabolism

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