Using Lipidomics Technology to Investigate the Regulatory Impact of Jianpi Lishi Formula on Lipid Metabolism in NAFLD Mice

  OBJECTIVE  To explore the regulatory effect of Jianpi Lishi Formula on liver lipid metabolism in NAFLD mice based on LC-MS lipidomics.

  METHODS  C57BL/6J mice were randomly divided into three groups: blank group, model group, and Jianpi Lishi Formula group, with 6 mice in each group. NAFLD models were induced by feeding them 60% high-fat feed for 12 weeks, during which medication intervention was also performed. The pharmacodynamic evaluation was conducted through biochemical indicator detection and pathological sections, while LC-MS lipidomics was used to detect lipid changes in mouse liver tissue.

  RESULTS  Jianpi Lishi Formula improved the increase in body mass induced by HFD. HE and oil red staining showed that it had a liver protective effect and could restore glucose metabolism levels. Also, Jianpi Lishi Formula decreased the mRNA levels of Fasn, CD36, SCD1, and Srebp1c in the liver (P < 0.05, P < 0. 01, P < 0. 001). Lipidomics research found that Jianpi Lishi Formula has a regulatory effect on liver lipid composition.

  CONCLUSION  Jianpi Lishi Formula has the effects of reducing body mass, regulating blood sugar, and protecting the liver. Lipidomics study indicates that the relief of NAFLD induced by Jianpi Lishi Formula may be related to the regulation of liver lipid composition.