OBJECTIVE To investigate the effect of Platycodon grandiflorum polysaccharides on regulating the growth and metabolism of Akkermansia muciniphila (A.muciniphila).
METHODS Platycodon grandiflorum polysaccharides were co-incubated with A.muciniphila in vitro and the growth curve was determined; Use LC-MS/MS to determine the content of short-chain fatty acids(SCFAs); Non-targeted metabolomics and non-targeted lipidomics were performed to study the changes of metabolic profiles in A.muciniphila after Platycodon grandiflorum polysaccharides treatment.
RESULTS Platycodon grandiflorum polysaccharides could promote the growth of A.muciniphila, and increase the content of short-chain fatty acids in A.muciniphila, especially butyrate. Furthermore, Platycodon grandiflorum polysaccharides also remodeled the metabolic profiles of A.muciniphila, as 9 lipids and 18 other metabolites were regulated.
CONCLUSION Platycodon grandiflorum polysaccharides can enrich A.muciniphila and regulate its metabolism. This study provides a theoretical basis for harnessing Platycodon grandiflorum polysaccharides as prebiotics of A.muciniphila.
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