OBJECTIVE To develop a method for the determination of saponins, amino acids and nucleosides in roots and rhizomes of Panax japonicus by UFLC-QTRAP-MS/MS.
METHODS The extract was separated on a XBridge®C18(4.6 mm×100 mm, 3.5 μm) at 30 ℃ with a gradient elution of 0.1% formic acid solution -0.1% formic acid acetonitrile, and the flow rate was 0.8 mL·min-1, using multiple-reaction monitoring (MRM) mode. Hierarchical cluster, nonlinear mapping and principal component analysis were adopted for comparison of roots and rhizomes.
RESULTS 33 constituents showed good linearity (r>0.999 0) in the range of the tested concentration; the precision, repeatability and stability were good; the average recovery rates were between 96.90% and 101.6%, the relative standard deviations were less than or equal to 3.6%.
CONCLUSION The results of data analysis showed that roots were similar to rhizomes according to contents of 33 constituents. The established method was accurate and reliable, which could used as a reference for quality evaluation and comprehensive control of Panax japonicus, and provided basic information for the development and utilization of roots of Panax japonicus.
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