OBJECTIVE To develop a biological evaluation method based on the blood tonic activity, combined with the main medicinal effects of Angelica sinensis, to evaluated its internal quality.
METHODS Hemin was used as standard substance, the relative content of hemoglobin after added Angelica sinensis to K562 cells was determined by the benzidine staining method and the biological titer was calculated by parallel-line assay. After methodological investigation, the biological titers of different production areas and processing of Angelica sinensis were compared.
RESULTS The reliability test of Angelica sinensis showed that the regression items were significantly different (P < 0.01). The RSDs of repeatability and precision were 1.13% and 4.73%, respectively, in the methodological investigation. The blood-enriching titers of its pieces from different origins were in the range of 399.61-8 920.32 U·mg-1. The rank of blood tonic effects of different processed Angelica sinensis products was raw > soil > wine, and that of different medicinal parts was body > head > tail.
CONCLUSION The established biological titer method of blood tonic for Angelica sinensis is stable and reliable. The results of methodological investigation are in accord with the relevant requirements of the "Guidelines for the determination of biological activity of traditional Chinese medicines" in the Chinese Pharmacopoeia (2020 edition). It can be used to evaluate the blood tonic titer of Angelica sinensis from different areas, processing methods and medicinal parts, which provides a new idea for further study on the improving of Angelica sinensis quality control.
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