Study on the Percutaneous Absorption Behavior of Compound Nanxing Zhitong Plaster Based on Different Skin Model

  OBJECTIVE  To evaluate the biopharmaceutical properties of five active ingredients in Compound Nanxing Zhitong Plaster (CNZP)by composite phospholipid liposome-based artificial skin membrane (CPLASM).

  METHODS  The HPLC analysis method was established to simultaneously determine five active ingredients (cinnamic aldehyde, paeonol, eugenol, methyl salicylate and imperatorin) in CNZP. CPLASM were prepared and used to measure the in vitro transdermal absorption parameters of the five active ingredients from CNZP in comparison with Strat-M^TM, the marketed artificial skin membrane. The obtained parameters were further compared with those obtained by rat skin and porcine ear skin.

  RESULTS  The five active components can be simultaneously measured by HPLC analysis. Among the five active ingredients, eugenol and methyl salicylate were found to possess the best permeability. The apparent permeability coefficient correlation between CPLASM and porcine ear skin was determined to be 0.965 8 and 0.943 3 while that between Strat-M^TM and porcine ear skin was found to be 0.949 3 and 0.946 0, respectively.

  CONCLUSION  CPLASM can be used to evaluate the permeation behavior of plasters of Chinese medicine such as CNZP.