Pharmacokinetic Study of Skin and Blood of Sinomenine-Loaded Microemulsion Gel Based on Double Sites Simultaneous Microdialysis

DING Yang; QIAN Shan-shan; GUO Jian; GUI Shuang-ying

doi:10.14148/j.issn.1672-0482.2022.1050

Volume 38 Issue 11

Nov. 2022

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Journal of Nanjing University of traditional Chinese Medicine > 2022 > 38(11): 1050-1055. > DOI: 10.14148/j.issn.1672-0482.2022.1050

DING Yang, QIAN Shan-shan, GUO Jian, GUI Shuang-ying. Pharmacokinetic Study of Skin and Blood of Sinomenine-Loaded Microemulsion Gel Based on Double Sites Simultaneous Microdialysis[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(11): 1050-1055. DOI: 10.14148/j.issn.1672-0482.2022.1050

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Pharmacokinetic Study of Skin and Blood of Sinomenine-Loaded Microemulsion Gel Based on Double Sites Simultaneous Microdialysis

DING Yang^{1, 2, 3, 4,},
QIAN Shan-shan⁵,
GUO Jian^{1, 2, 3, 4},
GUI Shuang-ying^{1, 2, 3, 4, ,}

1.
College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
2.
Institute of Pharmaceutics, Anhui Academy of Chinese Medicine, Hefei 230012, China
3.
Engineering Technology Research Center of Modernized Pharmaceutics Anhui Education Department, Hefei 230012, China
4.
Anhui Province Key Laboratory of Pharmaceutical Preparation Technology and Application, Hefei 230012, China
5.
The Second Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230061, China

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Received Date: August 14, 2022
Available Online: November 18, 2022

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Abstract

Abstract

OBJECTIVE To investigate the pharmacokinetics of sinomenine-loaded microemulsion gel (SMG) by skin and blood microdialysis in rats, and explore the pharmacokinetic characteristics of SMG.
METHODS An UPLC method for the determination of sinomenine was established, and its specificity, linearity, precision and recovery rate were examined. The probe was implanted after the rats were anesthetized and depilated, then the sinomenine microemulsion gel was applied onto the bare abdominal skin of the rats. The microdialysis technique and UPLC were used to determine the concentration of the drug.
RESULTS The results showed that the established method for the determination of sinomenine microemulsion gel was well linear in the range of 0.5-20 μg·mL^-1, and the specificity, degree of precision and recovery rate of the chromatography all conformed to the examination requirements of microdialysis samples.The results of pharmacokinetic study showed that the C_max values of sinomenine in skin and blood were (10.91±3.05) and (6.74±1.91)μg·mL^-1, respectively. The T_max values were (180.00±39.80) and (239.98±40.85)min, respectively. And the AUC_0→t values were (736.51±45.30) and (426.43±30.18)μg·min·mL^-1.
CONCLUSION The sinomenine microemulsion gel plays a role in forming skin reservoir through percutaneous penetration, and can control the sustained release of drug.
- transdermal administration,
- sinomenine,
- microemulsion gel,
- microdialysis,
- probe,
- transdermal pharmacokinetics

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Pharmacokinetic Study of Skin and Blood of Sinomenine-Loaded Microemulsion Gel Based on Double Sites Simultaneous Microdialysis

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