Effect of Arisaema Cum Bile on Brain Tissue Injury and Inflammation in Febrile Seizures Model Mice

  OBJECTIVE  To study the efficacy of Arisaema Cum Bile on antagonizing brain tissue damage and inflammation in febrile seizures model mice.

  METHODS  Mice model of febrile seizures was established by using dry yeast suspension and pentylenetetrazol solution, and the effects of low, medium and high dose groups of Arisaema Cum Bile water extract on rectal temperature, convulsion latency and convulsion duration of model mice were investigated. HE staining was used to observe the morphology of mice hippocampal neurons. ELISA was used to detect the levels of serum cAMP and PGE₂. Western blot method was used to detect the protein expressions of COX-2, iNOS, GFAP and GABAAR in brain tissue. The qPCR method was used to detect inflammatory factors IL-1β, IL-6, TNF-α mRNA expression in hippocampus tissue.

  RESULTS  Compared with the model group, the high-dose Arisaema Cum Bile group could reduce the rectal temperature (P < 0.05), prolong the convulsion latency (P < 0.05), shorten the duration of convulsions (P < 0.01), and up-regulate the expression of GABAAR protein (P < 0.05); At the same time, it decreased the levels of serum cAMP and PGE₂ (P < 0.01), and improved the morphological changes of hippocampal neurons, down-regulated the expressions of COX-2, iNOS and GFAP protein in the brain tissue (P < 0.05), and decreased the expression levels of IL-1β, IL-6 and TNF-α mRNA in the hippocampus (P < 0.05).

  CONCLUSION  Arisaema Cum Bile has a good antipyretic convulsant effect, and its mechanism may be related to antipyretic and antagonizing brain tissue inflammation.