The Effect of Erzhiwan on Glucose Metabolism of Adipose Tissue in Postmenopausal Osteoporosis Model Rats

  OBJECTIVE  To explore the effect of Erzhiwan on glucose metabolism of adipose tissue in postmenopausal osteoporosis model rats.

  METHODS  The postmenopausal osteoporosis rat model was established by bilateral ovariectomy. All model rats were randomly divided into 4 groups: model group, alendronate sodium group, sodium fluoride group and Erzhiwan group, with 8 rats in each group. Another 8 rats were set in sham operation group. Each group was given normal saline (5 mL·kg^-1), alendronate sodium (1 mg·kg^-1), sodium fluoride(5 mg·kg^-1) and Erzhiwan (1.6 g·kg^-1) once a day for 8 weeks, respectively. The sham group was given the same intervention as the model group. Serum insulin was detected by ELISA. The expressions of Glut4, PGC-1α, Sirt1 mRNA and protein in adipose tissue were detected by qPCR and Western blot.

  RESULTS  Compared with the sham group, the serum insulin in the model group increased significantly, and the mRNA and protein expressions of Glut4, PGC-1α and Sirt1 in adipose tissue decreased significantly. Compared with the model group, the serum insulin in Erzhiwan group decreased significantly, and the mRNA and protein expressions of Glut4, PGC-1α and Sirt1 in adipose tissue increased significantly.

  CONCLUSION  Erzhiwan can activate Sirt1/PGC-1α/Glut4 to improve the disorder of glucose metabolism in adipose tissue under postmenopausal osteoporosis.