SUN Ling, SHEN Cun-si, LIAO Ying-zhao, SHAN Jin-jun. Brain Metabolomics Reveals the Antipyretic Effects of Combined Use of Ling-Zhu-San and Acetaminophen in Young Rats Using Gas Chromatography-Mass Spectrometry[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(5): 409-418. DOI: 10.14148/j.issn.1672-0482.2022.0409
Citation: SUN Ling, SHEN Cun-si, LIAO Ying-zhao, SHAN Jin-jun. Brain Metabolomics Reveals the Antipyretic Effects of Combined Use of Ling-Zhu-San and Acetaminophen in Young Rats Using Gas Chromatography-Mass Spectrometry[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(5): 409-418. DOI: 10.14148/j.issn.1672-0482.2022.0409
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Brain Metabolomics Reveals the Antipyretic Effects of Combined Use of Ling-Zhu-San and Acetaminophen in Young Rats Using Gas Chromatography-Mass Spectrometry

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    • Abstract
  •   OBJECTIVE  To investigate the antipyretic and metabolic effects of Ling-Zhu-San (LZS) and Acetaminophen (APAP) on young rats.
      METHODS  The changes of metabolite levels in rat brain were confirmed by GC-MS platform, multivariate statistical technique and pathway analysis. The changes of temperature and the contents of IL-1β and PGE2 in rats were investigated by temperature test and enzyme-linked immunosorbent assay.
      RESULTS  16 metabolites associated with TCA cycle, tryptophan metabolism, glutamate metabolism and arachidonic acid metabolism (P < 0.05, FC>1.5). These metabolites were considered as potential biomarkers of fever. And the concentration of these metabolites basically recovered to normal levels after combination therapy. LZS combined with APAP had the best antipyretic effect.
      CONCLUSION  These results suggest that the antipyretic effect of LZS combined with APAP on young rats may be related to its improvement of brain metabolic disorders.
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