Effect of Arisaematis Rhizoma Processed on the Contents of Lectin Protein and Calcium Oxalate Crystal as Toxic Components

  OBJECTIVE  The present study was aimed to investigate the effect of Arisaematis Rhizoma processed on the contents of endogenic toxic components and to establish the detection method.

  METHODS  HPLC was applied to determinate the content of calcium oxalate. The separation was developed on Megres C₁₈ column (250 mm×4.6 mm, 5 μm) by gradient elution with water containing 0.5% phosphoric acid as the moble phase at the flow rate of 0.8 mL·min^-1, the column temperature of 25 ℃, and the detection wavelength of 220 nm. Western blot was applied to detect the content of lectin protein.

  RESULTS  The contents of calcium oxalate of all batches of raw Arisaematis Rhizoma was 0.78%-2.33%, and average content was 1.43%;The contents of calcium oxalate of all batches of processed Arisaematis Rhizoma were 0.29%-0.98%, and average content was 0.60%; After processing, the decreasing amplitude of calcium oxalate were 25.6%-78.3%. No lectin protein was detected in all batches of processed Arisaematis Rhizoma.

  CONCLUSION  The contents of toxic components, lectin protein and calcium oxalate, were decreased in Arisaematis Rhizoma after processed. The detection method of lectin protein and calcium oxalate can be used to detect toxic components of multi-batch Arisaematis Rhizoma, and can provide reference for the establishment of toxicity control index of Arisaematis Rhizoma. It is stable and reliable that consider the limit of lectin protein as toxicity control index of Arisaematis Rhizoma.