Study on Processing Procedure of Wine-Processed Ligustri Lucidi Fructus Based on UPLC Characteristic Chromatogram and Multicomponent Content Determination

  OBJECTIVE  Ultra Performance Liquid Chromatography (UPLC) characteristic chromatogram and simultaneous quantitative analysis method were established to provide reference for the quality evaluation of wine-processed Ligustri Lucidi Fructus with different processing procedure.

  METHODS  ACQUITY UPLC BEH Shield RP₁₈ (100 mm×2.1 mm, 1.7 μm) chromatographic column was used, with acetonitrile (A)-aqueous solution (B) as flow phase by gradient elution. The flow rate was 0.3 mL · min^-1, column temperature was 30 ℃ and detection wavelength of 0-3 min was 284 nm, 3-25 min was 224 nm, sample injection volume was 1 μL. The quality evaluation of different processing procedure of wine-processed Ligustri Lucidi Fructus was conducted by chemical pattern recognition.

  RESULTS  UPLC characteristic chromatogram of Ligustri Lucidi Fructus and wine-processed Ligustri Lucidi Fructus were established, identifying 10 and 14 common peaks, respectively. No. 3, 4, 7, 9 peaks were identified as Salidroside, Echinacoside, Specnuezhenide, and Ligustroflavone G13, respectively, and the new component (No. 11 peak) were identified as 5-Hydroxymethylfurfural after processing with wine. Clustering analysis and main component analysis roughly divided the wine-processed Ligustri Lucidi Fructus samples with different processing procedures into two categories. Combined with the orthogonal partial least squares-discrimination analysis, five main marker components that caused the differences between the different processing procedure samples were found. Three index components were quantitatively analyzed, while the content of Salidroside increased with prolonged processing time, and the contents of specnuezhenide and Ligustroflavone G13 stabilized with prolonged processing time.

  CONCLUSIONS  The UPLC characteristic chromatogram and simultaneous quantitative methods established in this study are accurate and repetitive, which is of great significance for the quality control and overall evaluation of Ligustri Lucidi Fructus and the processing procedure of wine-processed Ligustri Lucidi Fructust.