Volume 37 Issue 3
May  2021
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LIN Xia, HUANG You, YANG Sha-sha, WEI Xin-yi, FU Chao-mei, LI Ru, ZHANG Zhen. Effect of Fuzi Lizhong Pill on Intestinal Flora of Spleen Yang Deficiency IBS-D Rats Based on High-Throughput Sequencing Technique[J]. Journal of Nanjing University of traditional Chinese Medicine, 2021, 37(3): 388-395. DOI: 10.14148/j.issn.1672-0482.2021.0388
Citation: LIN Xia, HUANG You, YANG Sha-sha, WEI Xin-yi, FU Chao-mei, LI Ru, ZHANG Zhen. Effect of Fuzi Lizhong Pill on Intestinal Flora of Spleen Yang Deficiency IBS-D Rats Based on High-Throughput Sequencing Technique[J]. Journal of Nanjing University of traditional Chinese Medicine, 2021, 37(3): 388-395. DOI: 10.14148/j.issn.1672-0482.2021.0388
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Effect of Fuzi Lizhong Pill on Intestinal Flora of Spleen Yang Deficiency IBS-D Rats Based on High-Throughput Sequencing Technique

  • State Key Laboratory of Southwestern Chinese Medicine Resources, Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China

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  • Received Date: June 05, 2020
  • Available Online: December 20, 2021
  • Published Date: May 09, 2021
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  • OBJECTIVE  To research the effect and mechanism of Fuzi Lizhong Pill (FLZP) on intestinal flora of spleen Yang deficiency IBS-D rats, and to explore the relationship between intestinal flora and spleen Yang deficiency in rats. METHODS  60 male SD rats were selected and randomly divided into normal group, model group, FLZP low dose group and FLZP high dose group. Rats in normal group were fed normally. The spleen Yang deficiency IBS-D model were established by using the combined factor modeling method of "irregular diet + tired excessive + senna lavage" for 21 days. After successful modeling, FLZP was administrated in rats of FLZP low and high dose groups for 21 days. Then feces samples were collected from rats. Total DNA was extracted from these feces samples. Primers were designed according to the V3-V4 region of bacterial 16S rRNA for amplification. Finally, Illumina Miseq platform was used for high-throughput sequencing. RESULTS  Compared with normal group, the relative abundance of Bacteroidetes, Bacteroidia and Muribaculaceae in model group significantly reduced (P < 0.05) and the relative abundance of Clostridiaceae, Clostridium_sensu_stricto_1, Turicibacter, Blautia and Lactobacillus in model group significantly increased (P < 0.01). Compared with model group, the relative abundance of Bacteroidetes, Bacteroidia and Muribaculaceae in FLZP low and high dose groups significantly increased (P < 0.05) and the relative abundance of Clostridiaceae, Clostridium_sensu_stricto_1, Turicibacter, Blautia in FLZP low and high dose groups significantly reduced (P < 0.01). The relative abundance of Lactobacillus increased in FLZP low and high dose groups, and significantly in FLZP high dose group (P < 0.05). CONCLUSION   FLZP can increase the relative abundance of probiotics Lactobacillus and recover the relative abundance of Bacteroidetes, Turicibacter and Blautia. The regulation of intestinal flora that is closely related to inflammation and immune system, is the important mechanism of FLZP in treating spleen Yang deficiency IBS-D diarrhea.
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  • [1]
    灵枢经[M]. 北京: 人民卫生出版社, 1963: 55, 69.
    [2]
    内经选读[M]. 上海: 上海科学技术出版社, 2018: 45-46.
    [3]
    赵健. 基于近20年中医期刊文献的肠易激综合征辨证用药规律研究[D]. 北京: 北京中医药大学, 2014.
    [4]
    廖玉婷. IBS-D中医证候分型特点及膳食纤维对IBS-D症状影响的临床研究[D]. 北京: 北京中医药大学, 2017.
    [5]
    时岱, 姜莉云. 腹泻型肠易激综合征的中医药治疗概况[J]. 中国民族民间医药, 2016, 25(20): 48-50. https://www.cnki.com.cn/Article/CJFDTOTAL-MZMJ201620016.htm
    [6]
    史振红. 肠易激综合征(腹泻型)中医证候学特点与分布规律研究[D]. 广州: 广州中医药大学, 2012.
    [7]
    丁姮月, 孙宏文. 肠道菌群与腹泻型肠易激综合征相关性的研究进展[J]. 中国微生态学杂志, 2019, 31(1): 119-125. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGWS201901028.htm
    [8]
    刘佳星, 王彦礼, 李彧, 等. 四神丸对腹泻型肠易激综合征大鼠肠道菌群影响的实验研究[J]. 药学学报, 2019, 54(4): 670-677. https://www.cnki.com.cn/Article/CJFDTOTAL-YXXB201904011.htm
    [9]
    DISTRUTTI E, MONALDI L, RICCI P, et al. Gut microbiota role in irritable bowel syndrome: New therapeutic strategies[J]. World J Gastroenterol, 2016, 22(7): 2219-2241. DOI: 10.3748/wjg.v22.i7.2219
    [10]
    计敏, 黄华, 蓝显明, 等. 不同亚型肠易激综合征患者肠道菌群改变的观察[J]. 临床消化病杂志, 2016, 28(2): 103-106. https://www.cnki.com.cn/Article/CJFDTOTAL-LCXH201602016.htm
    [11]
    BÄCKHED F, LEY RE, SONNENBURG JL, et al. Host-bacterial mutualism in the human intestine[J]. Science, 2005, 307(5717): 1915-1920. DOI: 10.1126/science.1104816
    [12]
    NICHOLSON JK, HOLMES E, WILSON ID. Gut microorganisms, mammalian metabolism and personalized health care[J]. Nat Rev Microbiol, 2005, 3(5): 431-438. DOI: 10.1038/nrmicro1152
    [13]
    SOMMER F, ANDERSON JM, BHARTI R, et al. The resilience of the intestinal microbiota influences health and disease[J]. Nat Rev Microbiol, 2017, 15(10): 630-638. DOI: 10.1038/nrmicro.2017.58
    [14]
    徐航宇, 王彦礼, 王敦方, 等. 高通量测序技术研究黄芩汤对溃疡性结肠炎大鼠肠道菌群的影响[J]. 药学学报, 2017, 52(11): 1673-1682. https://www.cnki.com.cn/Article/CJFDTOTAL-YXXB201711006.htm
    [15]
    江茂源, 张臻, 石金凤, 等. 基于甘草中2种成分同时测定的附子理中丸溶出行为研究[J]. 中国中药杂志, 2018, 43(5): 952-958. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201805016.htm
    [16]
    郑超伟, 李建锋, 刘礼剑, 等. 附子理中丸联合当归生姜羊肉汤治疗脾肾阳虚证腹泻型肠易激综合征临床观察[J]. 中国中西医结合消化杂志, 2019, 27(3): 175-178. https://www.cnki.com.cn/Article/CJFDTOTAL-ZXPW201903004.htm
    [17]
    叶蜀晖. 四神丸合附子理中丸治疗腹泻型肠易激综合征62例[J]. 山东中医杂志, 2010, 29(5): 310. https://www.cnki.com.cn/Article/CJFDTOTAL-SDZY201005011.htm
    [18]
    孟莉, 郭新春. 附子理中丸联合益生菌治疗腹泻型肠易激综合征45例[J]. 现代中医药, 2019, 39(1): 70-72. https://www.cnki.com.cn/Article/CJFDTOTAL-XDZY201901026.htm
    [19]
    国家药典委员会. 中华人民共和国药典: 一部[S]. 北京: 中国医药科技出版社, 2015.
    [20]
    任培培, 汪龙德, 刘俊宏, 等. 腹泻型肠易激综合征肝郁脾虚型病证结合大鼠模型的研究方法[J]. 中医研究, 2019, 32(6): 49-52. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYYJ201906020.htm
    [21]
    武静, 王慧. 大建中汤对脾阳虚腹痛大鼠大脑皮质三磷酸腺苷含量和三磷酸腺苷酶活性的影响[J]. 解剖学杂志, 2017, 40(2): 168-170, 175. https://www.cnki.com.cn/Article/CJFDTOTAL-JPXZ201702014.htm
    [22]
    潘新, 胡昌江, 耿媛媛, 等. 脾肾阳虚泄泻大鼠模型造模方法研究[J]. 中国中药杂志, 2014, 39(23): 4658-4663. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201423034.htm
    [23]
    尚冰, 丛培玮, 许南阳, 等. 对脾阳虚状态下自由基损伤变化的探究[J]. 中华中医药学刊, 2013, 31(8): 1651-1652. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYHS201308027.htm
    [24]
    翟美丽, 于漫, 刘俊兰, 等. 附子理中汤对脾阳虚大鼠脂质过氧化损伤和能量代谢障碍的影响[J]. 中华中医药学刊, 2017, 35(11): 2889-2891. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYHS201711042.htm
    [25]
    吉琳, 申琪, 郭向东. 阳虚动物模型及检测指标的研究进展[J]. 中国比较医学杂志, 2021, 31(1): 138-145. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGDX202101021.htm
    [26]
    宁晚玲, 唐汉庆, 刘燕平, 等. 附子理中汤对脾阳虚大鼠AQP4-ANP-pGC轴的影响[J]. 中国实验方剂学杂志, 2017, 23(20): 126-131. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX201720023.htm
    [27]
    RUSU F, DUMITRASCU DL. Epidemiology of irritable bowel syndrome in the former communist countries from Eastern Europe: A systematic review[J]. Clujul Med, 2015, 88(2): 146-151. http://pdfs.semanticscholar.org/ea06/a56f0d7539c72626bc3d135ca22f2d851bd3.pdf
    [28]
    PEI LX, CHEN H, GUO J, et al. Effect of acupuncture and its influence on visceral hypersensitivity in IBS-D patients[J]. Medicine, 2018, 97(21): e10877. http://www.zhangqiaokeyan.com/academic-journal-foreign-pmc_detail_thesis/040005254353.html
    [29]
    敖丽丽, 李晔. 益肠止泻方治疗腹泻型肠易激综合征50例[J]. 中国实验方剂学杂志, 2015, 21(2): 213-216. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX201502056.htm
    [30]
    SU X, TANG Y, ZHANG J, et al. Curative effect of warming kidney and fortifying spleen recipe on diarrhea-predominant irritable bowel syndrome[J]. J Tradit Chin Med, 2013, 33(5): 615-619. http://www.onacademic.com/detail/journal_1000036185010110_9a31.html
    [31]
    COLLINS SM. A role for the gut microbiota in IBS[J]. Nat Rev Gastroenterol Hepatol, 2014, 11(8): 497-505. http://download.xuebalib.com/1iv7DVmTbyHE.pdf
    [32]
    齐翠娟. 肠道菌群在1型糖尿病患者的结构特征及潜在致病机制研究[D]. 北京: 北京协和医学院, 2016.
    [33]
    PRESLEY LL, WEI B, BRAUN J, et al. Bacteria associated with immunoregulatory cells in mice[J]. Appl Environ Microbiol, 2010, 76(3): 936-941. http://www.onacademic.com/detail/journal_1000037467731410_0a22.html
    [34]
    MATSUDA JL, MALLEVAEY T, SCOTT-BROWNE J, et al. CD1d-restricted iNKT cells, the 'Swiss-Army knife' of the immune system[J]. Curr Opin Immunol, 2008, 20(3): 358-368. http://www.onacademic.com/detail/journal_1000034583714810_5d52.html
    [35]
    桑婷婷, 郭铖洁, 郭丹丹, 等. 中医药通过调节肠道菌群抑制肥胖和炎症相关疾病的进展研究[J]. 中国中药杂志, 2018, 43(16): 3235-3242. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201816002.htm
    [36]
    李旻. 人体肠道菌群结构与宿主代谢的相关性研究[D]. 上海: 上海交通大学, 2009.
    [37]
    ANAS A, VAN DER POLL T, DE VOS AF. Role of CD14 in lung inflammation and infection[J]. Crit Care, 2010, 14(2): 209. DOI: 10.1186/cc8850
    [38]
    唐京, 陈明, 柯文灿, 等. 乳酸菌在疾病防治和人体保健中的应用研究进展[J]. 微生物学杂志, 2017, 37(4): 98-107. https://www.cnki.com.cn/Article/CJFDTOTAL-WSWX201704014.htm
    [39]
    王扬. Lactobacillus rhamnosus GG来源蛋白上调肠道IgA产生的机制研究[D]. 天津: 天津医科大学, 2016.
    [40]
    陈路芳. 乳酸杆菌制剂对脓毒症小鼠的保护作用及其肠道微生态与血清、粪便代谢组学研究[D]. 杭州: 浙江大学, 2017.
    [41]
    THOMAS CM, VERSALOVIC J. Probiotics-host communication[J]. Gut Microbes, 2010, 1(3): 148-163. http://europepmc.org/abstract/med/20672012
    [42]
    王东旭, 林连捷, 林艳, 等. 益生菌对腹泻型肠易激综合征患者疗效及肠道菌群的影响[J]. 中国现代医学杂志, 2015, 25(27): 67-70. https://www.cnki.com.cn/Article/CJFDTOTAL-ZXDY201527016.htm
    [43]
    陈伟琴. 益生菌联合奥替溴铵对腹泻型肠易激综合征患者的疗效及肠道菌群的影响[J]. 中国中西医结合消化杂志, 2017, 25(9): 671-674. https://www.cnki.com.cn/Article/CJFDTOTAL-ZXPW201709010.htm
    [44]
    DIDARI T, MOZAFFARI S, NIKFAR S, et al. Effectiveness of probiotics in irritable bowel syndrome: Updated systematic review with meta-analysis[J]. World J Gastroenterol, 2015, 21(10): 3072-3084. http://qikan.cqvip.com/Qikan/Article/Detail?id=90888889504849534948485056
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