Effect of Electroacupuncture on Regulation of Serum Inflammatory Factors and Nerve Cytokines and Nerve Function in PSD Rats

  OBJECTIVE  To investigate the effect of electroacupuncture on the regulation of serum inflammatory factors and neurocytokines and nerve function in PSD rats.

  METHODS  SD rats were divided into sham operation group, post-stroke depression group, electroacupuncture group and fluoxetine group. Middle cerebral artery occlusion(MCAO)model was established. After the operation, chronic unpredictable mild stimulation (CUMS) was performed for 4 weeks. The PSD model was established and electroacupuncture was performed simultaneously. After treatment, conduct behavioral testing, TTC staining, HE staining, serum inflammatory factors, neurotrophic.

  RESULTS  Compared with the sham operation group, the brain tissue infarct area was significantly increased after MCAO operation, and the neurological function defect was obvious; HE staining clearly showed that the MCAO group was seriously injured. After PSD modeling, compared with the sham operation group, the consumption of sucrose was significantly reduced, the time of forced swimming immobility was significantly prolonged, and there was no significant change in the horizontal and vertical movement distance; The content of inflammatory factors IL-1β, IL-6, TNF-α increased; the content of neurotrophic factors BDNF, IGF-1, and NGF decreased; the Nissl body was obviously missing after Nissl staining. Compared with the post-stroke depression group, the consumption of sucrose in the electroacupuncture group and the fluoxetine administration group was significantly increased, the time of forced swimming immobility was significantly shortened, and the horizontal and vertical movement distances did not change significantly, the content of inflammatory factors IL-1β, IL-6, TNF-α decreased, the content of neurotrophic factors BDNF, IGF-1, and NGF increased, which was statistically significant.Nissl body increased significantly.

  CONCLUSION  Electroacupuncture can improve the behavioral changes related to depression in PSD model rats, and its mechanism may be related to regulating serum inflammatory factors, increasing neurotrophic factors, and promoting neuroprotection.